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Direction Read each question carefully, pick the best answer and record the answer on the answer sheet.

 

1. Which of the following statements concerning the active site of an enzyme is not correct?

a. an enzyme can have only one active site

b. substances other than substrates can bind to the active site.

c. it is the site where substrates binds to the enzyme

d. it is the site where catalysis occurs

e. the conformation of the active site can change

 

2. Which of the following factors would have an affect on the direction of an enzyme catalyzed reactions?

a. only substrate concentration

b. relative concentration of substrate and product

c. temperature

d. pH

e. only enzyme concentration

 

3. Enzymes increase the rate of a reaction by:

a. lowering the energy of activation of the reaction

b. changing endergonic reactions into exergonic reactions

c. lowering the free energy yield of the reaction

d. increasing the kinetic energy of the reaction

e. by affecting the direction of the reaction

 

4. Noncompetitive enzyme inhibitors do not have which of the following properties?

a. some can be normal metabolic intermediates

b. some can bind to allosteric sites on enzymes

c. some can reduce the affinity of enzyme for substrate

d. some have no effect on the affinity of enzyme for substrate.

e. some have a high affinity for the active site of the enzyme

 

 

5. Which of the following are the products of the light dependent reactions of photosynthesis in eucaryotes?

a. NADP, O2, ATP

b. NADH, O2, ATP

c. O2, NADP, ATP

d. O2, NADPH, ATP

e. ATP, NADP, H+

 

6. An enzyme that can fix CO2 into organic compounds is known as a .

a. kinase

b. carbonase

c. isomerase

d. decarboxylase

e. carboxylase

 

7. Photosynthetic pigments that are found in the light harvesting complex (antenna complex) are:

a. chlorophyll a

b. chlorophyll b and carotinoids

c. chlorophyll a, chlorophyll b and carotinoids

d. carotinoids

e. chlorophyll a and carotinoids

 

8. Which of the following does not occur during cyclic photophosphorylation?

a. the absorption of light by the PSI reaction center

b. the production of ATP

c. the absorption of light by the light harvesting complex

d. the reduction of NADP

e. oxidation-reduction reactions

 

9. Glycolysis refers to the conversion of____to_________

with the net production of _________ ATP per molecule of glucose.

a. glucose, pyruvate, 2 molecules

b. pyruvate, ethanol, 2 molecules

c. glucose, lactate, 2 molecules

d. pyruvate, lactate, 0 molecules

e. glucose, pyruvate, 4 molecules.

 

use the following figures to answer the next six (10-14) questions

The following figures concern the reaction shown below and the effect of various compounds on the rate of the reaction

 

10.From the diagram of the reaction and data presented in figures 1 - 4, how many chemically different substances bind to the active site of the enzyme phosphofructokinase?

a. 1

b. 5

c. 3

d. 4

e. 2

 

11. From the data presented in figures 1- 4, which of the following substances could be inhibitors of the enzyme phosphofructokinase?

a. citrate

b. ADP

c. fructose 1,6 bisphosphate

d. AMP

e. fructose 6 phosphate

 

12. From the results shown in figure 2, which of the following statements is probably not correct?

a. the presence of citrate reduces the maximum velocity of the reaction

b. citrate is probably a non competitive inhibitor of phosphofructokinase

c. citrate probably binds to the active site of the enzyme

d. citrate probably does not bind to the active site

e. citrate concentrations can reduce the rate of glycolysis.

 

13. From the results shown in figure 4, which of the following statements is probably not correct?

a. ADP does not change the maximum velocity of the reaction

b. ADP probably affects the affinity of phosphofructokinase for the substrate.

c. ADP concentrations may effect the rate of glycolysis

d. at low fructose 6 phosphate concentrations, ADP can increase the rate of the reaction.

e. ADP is probably a competitive inhibitor of the enzyme

 

 

 

14. From the reaction shown and the results shown in figure 1 - 4, which of the following statements is probably correct?

a. ADP is not a product of the reaction

b. neither ATP nor ADP binds to the enzyme phosphofructokinase

c. very high concentrations of ATP will reduce the rate of the reaction.

d. ADP is a product of the reaction and can also inhibit the reaction.

e. The binding of ATP to the enzyme is not cooperative.

 

15. Which of the following compounds is an intermediate of glycolysis and the Calvin-Benson cycle?

a. pyruvate

b. phosphoenolpyruvate

c. glucose 6 phosphate

d. glyceraldehyde phosphate

e. dihyroxyacetone phosphate

 

16. Which of the following is not a property of ribozymes?

a. they carry out endergonic reactions

b. they are composed of RNA

c. they bind substrate

d. they carry out catalysis

e. they carry out several different reactions

 

17. During the Krebs cycle:

a. electrons are extracted from water

b. NADPH is produced

c. some carbons that originate from glucose are converted to CO2

d. O2 is produced

e. O2 is consumed

 

18. In muscle cells, when O2 concentrations are deficient, glucose can be converted to:

a. lactate

b. CO2

c. acetylCoA

d. ethanol

e. succinate

 

Use the following figures to answer the next ten (19-28) questions

 

 

19. The areas in these organelles that have the highest H+ ion concentrations are:

a. 1 and 5

b. 3 and 6

c. 2 and 8

d. 3 and 5

e. 3 and 7

 

20. The oxidation-reduction reactions of the electron transport chains in these two organelles are located in regions:

a. 1 and 5

b. 2 and 6

c. 7 and 2

d. 8 and 2

e. 4 and 7

 

 

21. The ATP synthetases in these two organelles are located in regions:

a. 1 and 5

b. 6 and 3

c. 4 and 7

d. 4 and 8

e. 2 and 7

 

22. The Krebs cycle enzymes are located in region and the Calvin-Benson cycle enzymes are located in region .

a. 6 and 1

b. 1 and 6

c. 3 and 5

d. 7 and 4

e. 8 and 2

 

23. The proteins involved in the splitting of water are located in region and the proteins involved in the synthesis of water are located in region a. 7 and 4

b. 6 and 1

c. 2 and 8

d. 8 and 2

e. 4 and 7

 

24. NADPH is produced in region and NADH is produced in region .

a. 1 and 6

b. 3 and 7

c. 5 and 1

d. 6 and 3

e. 6 and 1

 

25. CO2 is fixed in region and CO2 is produced in region .

a. 3 and 6

b. 5 and 4

c. 1 and 6

d. 1 and 5

e. 8 and 2

 

26. ATP is produced by substrate level phosphorylation in region and ATP is used in region .

a. 1 and 6

b. 5 and 3

c. 6 and 1

d. 3 and 5

e. 7 and 4

 

27. NADPH is oxidized by enzymes located in region

while NADH is oxidized by enzymes located in region .

a. 6 and 2

b. 6 and 4

c. 1 and 7

d. 1 and 5

e. 3 and 7

 

28. Glyceraldehyde phosphate can be found in region

while pyruvate can be found in region .

a. 3 and 5

b. 6 and 1

c. 1 and 6

d. 5 and 3

e. a and 7

 

29. Which of the following is not a property of coenzymes?

a. they are involved in catalysis

b. they can bind to the active site of an enzyme

c. they are organic or inorganic molecules.

d. not every enzyme requires a coenzyme

e. they can be altered by catalysis

 

30. A competitive inhibitor of an enzyme:

a. will alter the affinity of enzyme for substrate

b. will denature the enzyme

c. will bind the active site of the enzyme

d. will bind to an allosteric site

e. will always be involved in feedback inhibition

 

 

use the following figure to answer the next two questions (31 and 32)

 

31. Which of the above enzymes could be isolated from the stomach?

a. enzyme 3

b. enzyme 2

c. enzyme 1

d. enzyme 1 and 3

e. enzyme 2 and 3

 

32. Which of the above enzymes could be isolated from lysosomes?

a. enzyme 1

b. enzymes 2 and 3

c. enzyme 3

d. enzyme 2

e. enzymes 1 and 3

 

 

33. Which of the following distinguishes C4 plants from C3 plants?

a. C4 plants have stomata but C3 plants do not.

b. only C3 plants have the enzyme ribulose bisphosphate carboxylase

c. only C4 plants have the enzyme ribulose bisphosphate carboxylase

d. C4 plants have two different enzymes that can fix CO2

e. only C4 plants have a vascular system

 

 

34. Most of the ATP made during cellular respiration is generated by:

a. glycolysis

b. direct synthesis of ATP during the Krebs cycle

c. substrate-level phosphorylation

d. oxidative phosphorylation

e. direct synthesis of ATP during the Calvin-Benson

cycle

 

35. In certain kinds of fat cells called brown fat cells, electrons travel down the electron transport chain of mitochondria without the simultaneous pumping of protons into the intermembrane space. In these cells which of the following would not occur?

a. synthesis of water

b. consumption of O2

c. oxidation of NADH

d. chemiosmosis

e. substrate level phosphorylation

 

 

 

 

 

 

An enzyme that can fix CO2 into organic compounds is known as a .

a. kinase

b. carbonase

c. isomerase

d. decarboxylase

e. carboxylase

 

7. Photosynthetic pigments that are found in the light harvesting complex (antenna complex) are:

a. chlorophyll a

b. chlorophyll b and carotinoids

c. chlorophyll a, chlorophyll b and carotinoids

d. carotinoids

e. chlorophyll a and carotinoids

 

8. Which of the following does not occur during cyclic photophosphorylation?

a. the absorption of light by the PSI reaction center

b. the production of ATP

c. the absorption of light by the light harvesting complex

d. the reduction of NADP

e. oxidation-reduction reactions

 

9. Glycolysis refers to the conversion of to

with the net production of of ATP per molecule of glucose.

a. glucose, pyruvate, 2 molecules

b. pyruvate, ethanol, 2 molecules

c. glucose, lactate, 2 molecules

d. pyruvate, lactate, 0 molecules

e. glucose, pyruvate, 4 molecules.

 

 

 

 

 

 

 

 

use the following figures to answer the next six (10-14) questions

The following figures concern the reaction shown below and the effect of various compounds on the rate of the reaction

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

10.From the diagram of the reaction and data presented in figures 1 - 4, how many chemically different substances bind to the active site of the enzyme phosphofructokinase?

a. 1

b. 5

c. 3

d. 4

e. 2

 

11. From the data presented in figures 1- 4, which of the following substances could be inhibitors of the enzyme phosphofructokinase?

a. citrate

b. ADP

c. fructose 1,6 bisphosphate

d. AMP

e. fructose 6 phosphate

 

12. From the results shown in figure 2, which of the following statements is probably not correct?

a. the presence of citrate reduces the maximum velocity of the reaction

b. citrate is probably a non competitive inhibitor of phosphofructokinase

c. citrate probably binds to the active site of the enzyme

d. citrate probably does not bind to the active site

e. citrate concentrations can reduce the rate of glycolysis.

 

13. From the results shown in figure 4, which of the following statements is probably not correct?

a. ADP does not change the maximum velocity of the reaction

b. ADP probably affects the affinity of phosphofructokinase for the substrate.

c. ADP concentrations may effect the rate of glycolysis

d. at low fructose 6 phosphate concentrations, ADP can increase the rate of the reaction.

e. ADP is probably a competitive inhibitor of the enzyme

 

 

 

14. From the reaction shown and the results shown in figure 1 - 4, which of the following statements is probably correct?

a. ADP is not a product of the reaction

b. neither ATP nor ADP binds to the enzyme phosphofructokinase

c. very high concentrations of ATP will reduce the rate of the reaction.

d. ADP is a product of the reaction and can also inhibit the reaction.

e. The binding of ATP to the enzyme is not cooperative.

 

15. Which of the following compounds is an intermediate of glycolysis and the Calvin-Benson cycle?

a. pyruvate

b. phosphoenolpyruvate

c. glucose 6 phosphate

d. glyceraldehyde phosphate

e. dihyroxyacetone phosphate

 

16. Which of the following is not a property of ribozymes?

a. they carry out endergonic reactions

b. they are composed of RNA

c. they bind substrate

d. they carry out catalysis

e. they carry out several different reactions

 

17. During the Krebs cycle:

a. electrons are extracted from water

b. NADPH is produced

c. some carbons that originate from glucose are converted to CO2

d. O2 is produced

e. O2 is consumed

 

18. In muscle cells, when O2 concentrations are deficient, glucose can be converted to:

a. lactate

b. CO2

c. acetylCoA

d. ethanol

e. succinate

 

Use the following figures to answer the next ten (19-28) questions

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

19. The areas in these organelles that have the highest H+ ion concentrations are:

a. 1 and 5

b. 3 and 6

c. 2 and 8

d. 3 and 5

e. 3 and 7

 

20. The oxidation-reduction reactions of the electron transport chains in these two organelles are located in regions:

a. 1 and 5

b. 2 and 6

c. 7 and 2

d. 8 and 2

e. 4 and 7

 

 

 

 

 

21. The ATP synthetases in these two organelles are located in regions:

a. 1 and 5

b. 6 and 3

c. 4 and 7

d. 4 and 8

e. 2 and 7

 

22. The Krebs cycle enzymes are located in region and the Calvin-Benson cycle enzymes are located in region .

a. 6 and 1

b. 1 and 6

c. 3 and 5

d. 7 and 4

e. 8 and 2

 

23. The proteins involved in the splitting of water are located in region and the proteins involved in the synthesis of water are located in region a. 7 and 4

b. 6 and 1

c. 2 and 8

d. 8 and 2

e. 4 and 7

 

24. NADPH is produced in region and NADH is produced in region .

a. 1 and 6

b. 3 and 7

c. 5 and 1

d. 6 and 3

e. 6 and 1

 

25. CO2 is fixed in region and CO2 is produced in region .

a. 3 and 6

b. 5 and 4

c. 1 and 6

d. 1 and 5

e. 8 and 2

 

26. ATP is produced by substrate level phosphorylation in region and ATP is used in region .

a. 1 and 6

b. 5 and 3

c. 6 and 1

d. 3 and 5

e. 7 and 4

 

27. NADPH is oxidized by enzymes located in region

while NADH is oxidized by enzymes located in region .

a. 6 and 2

b. 6 and 4

c. 1 and 7

d. 1 and 5

e. 3 and 7

 

 

 

 

 

 

 

28. Glyceraldehyde phosphate can be found in region

while pyruvate can be found in region .

a. 3 and 5

b. 6 and 1

c. 1 and 6

d. 5 and 3

e. a and 7

 

 

 

 

 

29. Which of the following is not a property of coenzymes?

a. they are involved in catalysis

b. they can bind to the active site of an enzyme

c. they are organic or inorganic molecules.

d. not every enzyme requires a coenzyme

e. they can be altered by catalysis

 

30. A competitive inhibitor of an enzyme:

a. will alter the affinity of enzyme for substrate

b. will denature the enzyme

c. will bind the active site of the enzyme

d. will bind to an allosteric site

e. will always be involved in feedback inhibition

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

use the following figure to answer the next two questions (31 and 32)

 

31. Which of the above enzymes could be isolated from the stomach?

a. enzyme 3

b. enzyme 2

c. enzyme 1

d. enzyme 1 and 3

e. enzyme 2 and 3

 

32. Which of the above enzymes could be isolated from lysosomes?

a. enzyme 1

b. enzymes 2 and 3

c. enzyme 3

d. enzyme 2

e. enzymes 1 and 3

 

 

33. Which of the following distinguishes C4 plants from C3 plants?

a. C4 plants have stomata but C3 plants do not.

b. only C3 plants have the enzyme ribulose bisphosphate carboxylase

c. only C4 plants have the enzyme ribulose bisphosphate carboxylase

d. C4 plants have two different enzymes that can fix CO2

e. only C4 plants have a vascular system

 

 

34. Most of the ATP made during cellular respiration is generated by:

a. glycolysis

b. direct synthesis of ATP during the Krebs cycle

c. substrate-level phosphorylation

d. oxidative phosphorylation

e. direct synthesis of ATP during the Calvin-Benson

cycle

 

35. In certain kinds of fat cells called brown fat cells, electrons travel down the electron transport chain of mitochondria without the simultaneous pumping of protons into the intermembrane space. In these cells which of the following would not occur?

a. synthesis of water

b. consumption of O2

c. oxidation of NADH

d. chemiosmosis

e. substrate level phosphorylation