To understant the relationship between substrate concentration, product concentration and enzyme concentration consider the following situation.


Keq is a small number

(90% of 0.01M) is

0.9 X 0.01 = .009 M (enz-sub complex)

.001 M free enzyme and .009 M enz-sub complex

Keq = (.001)(.999)/.099 = .001M

At equilibrium, if most of the enzyme is in complex with substrate the the Keq is a reatively small number.


Keq is a large number

10% of 0.01M is

0.1 X 0.01 = .001 (enz-sub complex)

Keq = (.009)(.999)/.001 = .8991M

Very little of the enzyme is in complex with substrate and the Keq is a relatively large number.




Thus one can extrapolate and conclude, under very simplifying conditions


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