Your name_______________________

your social security number _______________________


Directions: read each question carefully, pick the best answer and record it on the answer sheet.


1. Gene A and gene B are known to be 10 map units apart. Individuals homozygous dominant for these genes are mated with homozygous recessive. The offspring are then mated with homozygous recessive individuals. If there are 1000 offspring from this cross, how many of the offspring would you expect to show the crossover phenotype?

a. 10

b. 50

c. 100

d. 250

e. 500


2. Cystic fibrosis is inherited as a simple recessive. Suppose a normal woman who carries the gene for the trait marries a normal man whose genotype is unknown. What percent of their children would be expected to have the disease

a. O %

b. 25%

c. 33%

d. 50%

e. It is impossible to determine from the information provided



6. Retroviruses have...

a. DNA as their genetic material

b. Protein as their genetic material

c. Double stranded RNA as their genetic material

d. (-) single stranded RNA as their genetic material

e. (+) single stranded RNA as their genetic material.


7. Pick the incorrect ending to the following:

Herpes viruses...

a. Can remain latent in muscle cells

b. Have DNA as their genetic material

c. Can infect epithelial cells

d. Can cause recurring infections

e. Have been shown to be associated with some types of cancers


8. At metaphase of mitosis each human chromosome has __ chromatids with each chromatid having ___ DNA as the corresponding chromosome in G1 of the cell cycle.

a. 1; twice the amount of

b. 2; the same amount of

c. 0; the same amount of

d. 2; twice the amount of

e. 4; the same amount of


9. Crossing over most commonly occurs during

a. Prophase I

b. Anaphase I

c. Interphase

d. Prophase II

e. Telophase II.


10. A diploid human male cell has 46 chromosomes and a diploid human female cell has 46 chromosomes. A human cell containing 22 autosomes and a Y chromosome is

a. A somatic cell of a male

b. A zygote

c. A somatic cell of a female

d. A sperm cell

e. An ovum


Use the following figure to answer the next four (11-14) questions


11. The nitrogenous base that is normally found in RNA but not DNA is:

a. 5 (guanine)

b. 1 (ribose)

c. 3 (adenine)

d. 4 (thymine)

e. 7 (uracil)


12. The sugar found in DNA but not RNA is:

a. 1

b. 2

c. 3

d. 4

e. 5


13. The sugar found in RNA but not DNA is:

a. 1

b. 2

c. 3

d. 4

e. 5


14. Ultra Violet light can cause covalent modifications of which of the following:

a. 1

b. 2

c. 3

d. 4

e. 5


15. During S phase, chromosomal DNA is replicated in the:

a. 5' to 3' direction

b. 3' to 5' direction

c. 5' to 3' and 3' to 5' direction


16. Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

a. Restriction enzyme - production of RFLPs

b. DNA ligase - enzyme that cuts DNA, creating the sticky ends of reaction fragments

c. DNA polymerase - used in a polymerase chain reaction to amplify sections of DNA.

d. Reverse transcriptase - production of DNA from an mRNA template

e. Electrophoresis - DNA sequencing


17. In recombinant DNA methods, the term vector refers to

a. The enzyme that cuts DNA into restriction fragments

b. The sticky end of a DNA fragment

c. A RFLP marker

d. A plasmid or other agent used to transfer DNA into a living cell

e. A DNA probe used to identify a particular gene



18. The hepatitus B viruse and the retroviruses

a. Are both (+) single stranded RNA viruses

b. Both use reverse transcriptase in their reproduction

c. Both infect the same cell types

d. Both are double stranded DNA viruses

e. Both lead to AIDS


19. Pick the correct ending to the following: Introns are..

a. Protected by men in black

b. Not found in mRNA sequences

c. Are not found in eukaryotic genes

d. Are not found in nuclear RNA

e. Contain nitrogenous bases


20. Pick the incorrect ending to the following: The influenza virus;

a. Can infect more than one species

b. Has RNA as its genetic material

c. Genetic material is copied into DNA in the cytoplasm of an infected cell

d. Does not use reverse transcriptase during its replication

e. Is responsible for periodic pandemics


21. A promotor is

a. The site where DNA replication starts

b. The repressor binding site

c. The RNA polymerase binding site

d. A compound that induces RNA synthesis

e. A compound that blocks RNA synthesis


22. A nonsense mutation in the gene that codes for the repressor of the lactose operon would...

a. Block transcription of the lactose operon

b. Blocks RNA polymerase binding to the operator

c. Blocks glucose metabolism

d. Blocks production of cAMP

e. Results in an inactive repressor



23. Which of the following statements concerning mutations is not correct?

a. Mutations are changes in the genetic material of an organism

b. UV light and X-rays cause mutations by the same mechanism

c. Point mutations are changes in a single base.

d. Ethidium bromide can cause frame shift mutations

e. Some mutagens cause mutations by physically altering the DNA


24. Pick the incorrect ending to the following: A missense mutation...

a. Can result from exposure to UV light

b. Can result in no phenotypic change to the organism

c. Can result in death of the organism

d. Can drastically alters the size of the protein coded for by the gene which has the mutation

e. Can result in an altered primary structure of the protein coded for by the gene having the mutatio


25. To insert a "foreign" piece of DNA into a specific site on a plasmid and to make billions of copies of the recombinant plasmid, all of the following are required except.

a. Restriction enzyme

b. Polymerase chain reaction

c. Bacterial transformation

d. DNA ligase

e. Plasmid isolation techniques


use the following list to answer the next two questions (26-28)

1. Is usually single stranded

2. Contain pyrimidines

3. Contains deoxyribose

4. Is usually coiled in a double helix

5. It contains uracil

6. Contains adenine

7. Is involved in transcription

8. Is involved in translation

9 is present in or on a ribosome


26. DNA ...

a. 1, 4, 5 and 8

b. 2, 3, 7 and 4

c. 2, 3, 7 and 8

d. 1, 2, 3 and 7


27. RNA ..

a. 1, 2, 9 and 7

b. 1, 2, 7 and 4

c. 2, 7, 6 and 4

d. 9, 8, 4 and 1

e. 9, 7, 4 and 1


28. Given the genotype AaBb, what fraction of the gametes will be genotypically AB

a. 1/4

b. 1/2

c. It is not possible to determine without more information.

d. 1/1

e. 1/8




29. A certain form of red-green color blindness is the result of an X chromosome-linked recessive allele. If a man is color blind and his wife has normal vision but her father was colorblind, could the man and woman have a daughter that was colorblind?

a. Yes

b. No

c. It is impossible to determine with the information provided

d. Yes, and the probability is 10%

e. Yes and the probability is 1/8.



30. Which of the following events would not occur if wild type E. Coli cells were grown in the presence of lactose?

a. The repressor of the lactose operon would be synthesized

b. RNA polymerase would bind to the lactose operon promotor.

c. The inducer would bind to the repressor

d. The gene coding for beta galactosidase would be transcribed

e. The repressor of the lactose operon would bind to the operator.



Use the following information to answer the next four questions. Below you will see an example of an gel in which the results of Sanger DNA sequencing reactions are shown. The DNA that was sequenced was the antisense strand of a gene coding for a human brain peptide hormone having 10 amino acids.


31. The first (N terminal) amino acid of the mature peptide hormone is meth and the last (C terminal) amino acid is phenylalanine. The third amino acid from the N-terminal end of the mature peptide hormone is alanine. The codon used for this amino acid is ____.

a. GCU

b. GCC

c. GCA

d. GCG

e. GGG


32. Certain individuals who have obsessive-compulsive disorder have missense mutations in the gene coding for this hormone. In these individual the hormone coded for by the mutated gene would have _______ amino acids.

a. 10

b. 11.

c. 7

d. 3

e. It is impossible to determine given the information provided


33. A geneticist wanted to induce point mutations in this gene by Ultra Violet (UV) light mutagenesis. Do you think it will work?

a. Yes

b. No


34. A famous researcher claimed that she had induced a frame shift mutation in this gene. The protein produced by the mutated gene had only 4 amino acids. The researcher deduced that the frame shift mutation produced a premature stop translation codon. Do you think this is possible?

a. Yes

b. No

c. Not enough information is provided to answer this question.


35. Which of the following is not correct concerning this gene?

a. It has more guanines than cytosines

b. It has more thymines than adenines

c. The translation process for this gene does not start with the codon AUG

d. The gene has 33 bases if one includes the start and stop codons

e. The gene does not contain any introns.