Home Up Introduction Structural Characterization Catalytic Mechanism Enzyme Evolution References



Bonner, G., Lafer, E. M., and Sousa, R. (1994). The thumb subdomain of T7 RNA polymerase functions to stabilize the ternary complex during processivity transcription.   J. Biol. Chem. 269, 25129-25136 [Medline] [Free Full Text].

Bonner, G., Patra, D. Lafer, E. M., and Sousa, R. (1992).  Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure.    EMBO J. 11, 3767-3775 [Medline].

Brieba, L. G. and Sousa, R. (2000).  Roles of Histidine 784 and Tyrosine 639 in ribose discrimination by T7 RNA polymerase. Biochemistry 39, 919-923. [Medline] [Full Text for CUNY].

Cheetham, G. M. T.,  Jeruzalmi, D., and Steitz, T. A (1999a).   Structural basis for initiation of transcription from an RNA polymerase-promoter complex.  Nature 399, 80-83 [Medline].

Cheetham, G. M. T. and  Steitz, T. A. (1999b). Structure of a transcribing T7 RNA polymerase initiation complex.  Science 286, 2305-2309 [Medline] [Free Full Text].

Dunn, J. J. and Studier, F. W. (1983).  Complete nucleotide sequence of bacteriophage T7 DNA.  J. Mol. Biol. 166, 477-536 [Medline].

Gao, G., Orlova, M.,Georgiadis, M. M, Hendrickson, W. A., and Goff, S. P. (1997).  Confering RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selectivity.   Proc. Natl. Acad. Sci. U.S.A. 94, 407-411 [Medline] [Free Full Text].

Jeruzalmi, D., and Steitz, T. A (1998). Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme.  EMBO J. 17, 4101-4113 [Medline] [Free Full Text].

Jia, Y. and Patel, S. S. (1997).  Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase.  Biochemistry 36, 4223-4232 [Medline] [Full Text for CUNY].

Joyce, C. (1997).  Choosing the right sugar: how polymerases selects a nucleotide substrate.  Proc. Natl. Acad. Sci. U.S.A. 94, 1619-1622 [Medline] [Free Full Text].

Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A., and Steitz, T. A. (1992). Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science, 256, 1783-1790 [Medline].

Moffatt, B. A., Dunn, J. J., and Studier, F. W. (1984).  Nucleotide sequence and the gene for bacteriophage T7 RNA polymerase.  J. Mol. Biol. 173, 265-269 [Medline].

Muller, D.K., Martin, C.T. and Coleman, J.E.  (1988).  Processivity of proteolytically modified forms of T7 RNA polymerase.  Biochemistry 27, 5763-5771 [Medline].

Ricchetti, M. and Buc, H.  (1993).  E. coli DNA polymerase I as a reverse transcriptase.  EMBO J.  12, 387-396 [Medline].

Severinov, K. (2001). T7 RNA polymerase transcription complex: What you see is not what you get. Proc. Natl. Acad. Sci. USA 98, 5-7 [Medline] [Full Text].

Sousa, R., Chung, Y. J., Rose, J. P. and Wang, B.-C. (1993).  Crystal structure of bacteriophage T7 RNA polymerase at 3.3 resolution.   Nature 364, 593-599 [Medline].

Sousa, R.  and Padilla, R. (1995).  A mutant T7 RNA polymerase as a DNA polymerase.  EMBO J. 14, 4609-4621 [Medline].

Steitz, T. A. (1998). A mechanism for all polymerases.  Nature, 391, 231-232 [Medline].

Steitz, T. A., Smerdon, S. J., Jager, J. and Joyce, C. (1994).  A unified polymerase mechanism for nonhomologous DNA and RNA polymerases.  Science 266, 2025-2026 [Medline].

Temiakov, D., Mentesana, P. E., Ma, K., Mustaev, A., Borukhov, S., and McAllister, W. T. (2000). The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance. Proc. Natl. Acad. Sci. USA 97, 14109-14114 [Medline] [Free Full Text].

Ujvari, A. and Martin, C. T. (1996). Thermodynamic and kinetic measurements of promoter binding by T7 RNA polymerase.  Biochemistry 35, 14574-14582 [Medline] [Full Text for CUNY].




I gratefully thank Dr. Manfred Philipp for directing the lectures of this enzymology class at The Graduate Center.  A whole variety of topics regarding enzyme catalysis were covered through the participation of successful investigators in the field of enzymology.  I would also thanks Dr. Philipp for providing me with an introductory insight into the field of bioinformatics.


[Back]  [Home]