References
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References:

Bonner, G., Lafer, E. M., and Sousa, R. (1994). The thumb subdomain of T7 RNA polymerase functions to stabilize the ternary complex during processivity transcription.   J. Biol. Chem. 269, 25129-25136 [Medline] [Free Full Text].

Bonner, G., Patra, D. Lafer, E. M., and Sousa, R. (1992).  Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure.    EMBO J. 11, 3767-3775 [Medline].

Brieba, L. G. and Sousa, R. (2000).  Roles of Histidine 784 and Tyrosine 639 in ribose discrimination by T7 RNA polymerase. Biochemistry 39, 919-923. [Medline] [Full Text for CUNY].

Cheetham, G. M. T.,  Jeruzalmi, D., and Steitz, T. A (1999a).   Structural basis for initiation of transcription from an RNA polymerase-promoter complex.  Nature 399, 80-83 [Medline].

Cheetham, G. M. T. and  Steitz, T. A. (1999b). Structure of a transcribing T7 RNA polymerase initiation complex.  Science 286, 2305-2309 [Medline] [Free Full Text].

Dunn, J. J. and Studier, F. W. (1983).  Complete nucleotide sequence of bacteriophage T7 DNA.  J. Mol. Biol. 166, 477-536 [Medline].

Gao, G., Orlova, M.,Georgiadis, M. M, Hendrickson, W. A., and Goff, S. P. (1997).  Confering RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selectivity.   Proc. Natl. Acad. Sci. U.S.A. 94, 407-411 [Medline] [Free Full Text].

Jeruzalmi, D., and Steitz, T. A (1998). Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme.  EMBO J. 17, 4101-4113 [Medline] [Free Full Text].

Jia, Y. and Patel, S. S. (1997).  Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase.  Biochemistry 36, 4223-4232 [Medline] [Full Text for CUNY].

Joyce, C. (1997).  Choosing the right sugar: how polymerases selects a nucleotide substrate.  Proc. Natl. Acad. Sci. U.S.A. 94, 1619-1622 [Medline] [Free Full Text].

Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A., and Steitz, T. A. (1992). Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science, 256, 1783-1790 [Medline].

Moffatt, B. A., Dunn, J. J., and Studier, F. W. (1984).  Nucleotide sequence and the gene for bacteriophage T7 RNA polymerase.  J. Mol. Biol. 173, 265-269 [Medline].

Muller, D.K., Martin, C.T. and Coleman, J.E.  (1988).  Processivity of proteolytically modified forms of T7 RNA polymerase.  Biochemistry 27, 5763-5771 [Medline].

Ricchetti, M. and Buc, H.  (1993).  E. coli DNA polymerase I as a reverse transcriptase.  EMBO J.  12, 387-396 [Medline].

Severinov, K. (2001). T7 RNA polymerase transcription complex: What you see is not what you get. Proc. Natl. Acad. Sci. USA 98, 5-7 [Medline] [Full Text].

Sousa, R., Chung, Y. J., Rose, J. P. and Wang, B.-C. (1993).  Crystal structure of bacteriophage T7 RNA polymerase at 3.3 resolution.   Nature 364, 593-599 [Medline].

Sousa, R.  and Padilla, R. (1995).  A mutant T7 RNA polymerase as a DNA polymerase.  EMBO J. 14, 4609-4621 [Medline].

Steitz, T. A. (1998). A mechanism for all polymerases.  Nature, 391, 231-232 [Medline].

Steitz, T. A., Smerdon, S. J., Jager, J. and Joyce, C. (1994).  A unified polymerase mechanism for nonhomologous DNA and RNA polymerases.  Science 266, 2025-2026 [Medline].

Temiakov, D., Mentesana, P. E., Ma, K., Mustaev, A., Borukhov, S., and McAllister, W. T. (2000). The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance. Proc. Natl. Acad. Sci. USA 97, 14109-14114 [Medline] [Free Full Text].

Ujvari, A. and Martin, C. T. (1996). Thermodynamic and kinetic measurements of promoter binding by T7 RNA polymerase.  Biochemistry 35, 14574-14582 [Medline] [Full Text for CUNY].

 

 

Acknowledgement:

I gratefully thank Dr. Manfred Philipp for directing the lectures of this enzymology class at The Graduate Center.  A whole variety of topics regarding enzyme catalysis were covered through the participation of successful investigators in the field of enzymology.  I would also thanks Dr. Philipp for providing me with an introductory insight into the field of bioinformatics.

 

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